Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids.
Identifieur interne : 003063 ( Main/Exploration ); précédent : 003062; suivant : 003064Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids.
Auteurs : Sridev Mohapatra [États-Unis] ; Rakesh Minocha ; Stephanie Long ; Subhash C. MinochaSource :
- Amino acids [ 1438-2199 ] ; 2010.
Descripteurs français
- KwdFr :
- Acide gamma-amino-butyrique (métabolisme), Acides aminés (métabolisme), Animaux (MeSH), Azote (analyse), Carbone (analyse), Facteurs temps (MeSH), Lignée cellulaire (MeSH), Ornithine (métabolisme), Ornithine decarboxylase (génétique), Perchlorates (MeSH), Populus (génétique), Populus (métabolisme), Putrescine (biosynthèse), Putrescine (métabolisme), Régulation positive (MeSH), Solubilité (MeSH), Souris (MeSH), Spermidine (métabolisme), Spermine (métabolisme), Statistiques comme sujet (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- analyse : Azote, Carbone.
- biosynthèse : Putrescine.
- génétique : Ornithine decarboxylase, Populus.
- métabolisme : Acide gamma-amino-butyrique, Acides aminés, Ornithine, Populus, Putrescine, Spermidine, Spermine.
- Animaux, Facteurs temps, Lignée cellulaire, Perchlorates, Régulation positive, Solubilité, Souris, Statistiques comme sujet, Transgènes, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Amino Acids (metabolism), Animals (MeSH), Carbon (analysis), Cell Line (MeSH), Mice (MeSH), Nitrogen (analysis), Ornithine (metabolism), Ornithine Decarboxylase (genetics), Perchlorates (MeSH), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (metabolism), Putrescine (biosynthesis), Putrescine (metabolism), Solubility (MeSH), Spermidine (metabolism), Spermine (metabolism), Statistics as Topic (MeSH), Time Factors (MeSH), Transgenes (MeSH), Up-Regulation (MeSH), gamma-Aminobutyric Acid (metabolism).
- MESH :
- chemical , analysis : Carbon, Nitrogen.
- chemical , biosynthesis : Putrescine.
- chemical , genetics : Ornithine Decarboxylase.
- chemical , metabolism : Amino Acids, Ornithine, Putrescine, Spermidine, Spermine, gamma-Aminobutyric Acid.
- genetics : Populus.
- metabolism : Populus.
- Animals, Cell Line, Mice, Perchlorates, Plants, Genetically Modified, Solubility, Statistics as Topic, Time Factors, Transgenes, Up-Regulation.
Abstract
The polyamine metabolic pathway is intricately connected to metabolism of several amino acids. While ornithine and arginine are direct precursors of putrescine, they themselves are synthesized from glutamate in multiple steps involving several enzymes. Additionally, glutamate is an amino group donor for several other amino acids and acts as a substrate for biosynthesis of proline and gamma-aminobutyric acid, metabolites that play important roles in plant development and stress response. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a constitutively expressing mouse ornithine decarboxylase gene, were used to study the effect of up-regulation of putrescine biosynthesis (and concomitantly its enhanced catabolism) on cellular contents of various protein and non-protein amino acids. It was observed that up-regulation of putrescine metabolism affected the steady state concentrations of most amino acids in the cells. While there was a decrease in the cellular contents of glutamine, glutamate, ornithine, arginine, histidine, serine, glycine, cysteine, phenylalanine, tryptophan, aspartate, lysine, leucine and methionine, an increase was seen in the contents of alanine, threonine, valine, isoleucine and gamma-aminobutyric acid. An overall increase in percent cellular nitrogen and carbon content was also observed in high putrescine metabolizing cells compared to control cells. It is concluded that genetic manipulation of putrescine biosynthesis affecting ornithine consumption caused a major change in the entire ornithine biosynthetic pathway and had pleiotropic effects on other amino acids and total cellular carbon and nitrogen, as well. We suggest that ornithine plays a key role in regulating this pathway.
DOI: 10.1007/s00726-009-0322-z
PubMed: 19649694
Affiliations:
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Minocha</name></author></analytic><series><title level="j">Amino acids</title><idno type="eISSN">1438-2199</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term><term>Animals (MeSH)</term><term>Carbon (analysis)</term><term>Cell Line (MeSH)</term><term>Mice (MeSH)</term><term>Nitrogen (analysis)</term><term>Ornithine (metabolism)</term><term>Ornithine Decarboxylase (genetics)</term><term>Perchlorates (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Putrescine (biosynthesis)</term><term>Putrescine (metabolism)</term><term>Solubility (MeSH)</term><term>Spermidine (metabolism)</term><term>Spermine (metabolism)</term><term>Statistics as Topic (MeSH)</term><term>Time Factors (MeSH)</term><term>Transgenes (MeSH)</term><term>Up-Regulation (MeSH)</term><term>gamma-Aminobutyric Acid (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide gamma-amino-butyrique (métabolisme)</term><term>Acides aminés (métabolisme)</term><term>Animaux (MeSH)</term><term>Azote (analyse)</term><term>Carbone (analyse)</term><term>Facteurs temps (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Ornithine (métabolisme)</term><term>Ornithine decarboxylase (génétique)</term><term>Perchlorates (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Putrescine (biosynthèse)</term><term>Putrescine (métabolisme)</term><term>Régulation positive (MeSH)</term><term>Solubilité (MeSH)</term><term>Souris (MeSH)</term><term>Spermidine (métabolisme)</term><term>Spermine (métabolisme)</term><term>Statistiques comme sujet (MeSH)</term><term>Transgènes (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbon</term><term>Nitrogen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Putrescine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Ornithine Decarboxylase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Ornithine</term><term>Putrescine</term><term>Spermidine</term><term>Spermine</term><term>gamma-Aminobutyric Acid</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Azote</term><term>Carbone</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Putrescine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ornithine decarboxylase</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide gamma-amino-butyrique</term><term>Acides aminés</term><term>Ornithine</term><term>Populus</term><term>Putrescine</term><term>Spermidine</term><term>Spermine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Mice</term><term>Perchlorates</term><term>Plants, Genetically Modified</term><term>Solubility</term><term>Statistics as Topic</term><term>Time Factors</term><term>Transgenes</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteurs temps</term><term>Lignée cellulaire</term><term>Perchlorates</term><term>Régulation positive</term><term>Solubilité</term><term>Souris</term><term>Statistiques comme sujet</term><term>Transgènes</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The polyamine metabolic pathway is intricately connected to metabolism of several amino acids. While ornithine and arginine are direct precursors of putrescine, they themselves are synthesized from glutamate in multiple steps involving several enzymes. Additionally, glutamate is an amino group donor for several other amino acids and acts as a substrate for biosynthesis of proline and gamma-aminobutyric acid, metabolites that play important roles in plant development and stress response. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a constitutively expressing mouse ornithine decarboxylase gene, were used to study the effect of up-regulation of putrescine biosynthesis (and concomitantly its enhanced catabolism) on cellular contents of various protein and non-protein amino acids. It was observed that up-regulation of putrescine metabolism affected the steady state concentrations of most amino acids in the cells. While there was a decrease in the cellular contents of glutamine, glutamate, ornithine, arginine, histidine, serine, glycine, cysteine, phenylalanine, tryptophan, aspartate, lysine, leucine and methionine, an increase was seen in the contents of alanine, threonine, valine, isoleucine and gamma-aminobutyric acid. An overall increase in percent cellular nitrogen and carbon content was also observed in high putrescine metabolizing cells compared to control cells. It is concluded that genetic manipulation of putrescine biosynthesis affecting ornithine consumption caused a major change in the entire ornithine biosynthetic pathway and had pleiotropic effects on other amino acids and total cellular carbon and nitrogen, as well. We suggest that ornithine plays a key role in regulating this pathway.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19649694</PMID><DateCompleted><Year>2010</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1438-2199</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Amino acids</Title><ISOAbbreviation>Amino Acids</ISOAbbreviation></Journal><ArticleTitle>Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids.</ArticleTitle><Pagination><MedlinePgn>1117-29</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00726-009-0322-z</ELocationID><Abstract><AbstractText>The polyamine metabolic pathway is intricately connected to metabolism of several amino acids. While ornithine and arginine are direct precursors of putrescine, they themselves are synthesized from glutamate in multiple steps involving several enzymes. Additionally, glutamate is an amino group donor for several other amino acids and acts as a substrate for biosynthesis of proline and gamma-aminobutyric acid, metabolites that play important roles in plant development and stress response. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a constitutively expressing mouse ornithine decarboxylase gene, were used to study the effect of up-regulation of putrescine biosynthesis (and concomitantly its enhanced catabolism) on cellular contents of various protein and non-protein amino acids. It was observed that up-regulation of putrescine metabolism affected the steady state concentrations of most amino acids in the cells. While there was a decrease in the cellular contents of glutamine, glutamate, ornithine, arginine, histidine, serine, glycine, cysteine, phenylalanine, tryptophan, aspartate, lysine, leucine and methionine, an increase was seen in the contents of alanine, threonine, valine, isoleucine and gamma-aminobutyric acid. An overall increase in percent cellular nitrogen and carbon content was also observed in high putrescine metabolizing cells compared to control cells. It is concluded that genetic manipulation of putrescine biosynthesis affecting ornithine consumption caused a major change in the entire ornithine biosynthetic pathway and had pleiotropic effects on other amino acids and total cellular carbon and nitrogen, as well. We suggest that ornithine plays a key role in regulating this pathway.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mohapatra</LastName><ForeName>Sridev</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Minocha</LastName><ForeName>Rakesh</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Stephanie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Minocha</LastName><ForeName>Subhash C</ForeName><Initials>SC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>08</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Amino Acids</MedlineTA><NlmUniqueID>9200312</NlmUniqueID><ISSNLinking>0939-4451</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010472">Perchlorates</NameOfSubstance></Chemical><Chemical><RegistryNumber>2FZ7Y3VOQX</RegistryNumber><NameOfSubstance UI="D013096">Spermine</NameOfSubstance></Chemical><Chemical><RegistryNumber>56-12-2</RegistryNumber><NameOfSubstance UI="D005680">gamma-Aminobutyric Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>E524N2IXA3</RegistryNumber><NameOfSubstance UI="D009952">Ornithine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.1.1.17</RegistryNumber><NameOfSubstance UI="D009955">Ornithine Decarboxylase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical><Chemical><RegistryNumber>U87FK77H25</RegistryNumber><NameOfSubstance UI="D013095">Spermidine</NameOfSubstance></Chemical><Chemical><RegistryNumber>V10TVZ52E4</RegistryNumber><NameOfSubstance UI="D011700">Putrescine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009952" MajorTopicYN="N">Ornithine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009955" MajorTopicYN="N">Ornithine Decarboxylase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010472" MajorTopicYN="N">Perchlorates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011700" MajorTopicYN="N">Putrescine</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013095" MajorTopicYN="N">Spermidine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013096" MajorTopicYN="N">Spermine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013223" MajorTopicYN="N">Statistics as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015854" MajorTopicYN="Y">Up-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005680" MajorTopicYN="N">gamma-Aminobutyric Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>06</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>8</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>8</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19649694</ArticleId><ArticleId IdType="doi">10.1007/s00726-009-0322-z</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>New Hampshire</li></region></list><tree><noCountry><name sortKey="Long, Stephanie" sort="Long, Stephanie" uniqKey="Long S" first="Stephanie" last="Long">Stephanie Long</name><name sortKey="Minocha, Rakesh" sort="Minocha, Rakesh" uniqKey="Minocha R" first="Rakesh" last="Minocha">Rakesh Minocha</name><name sortKey="Minocha, Subhash C" sort="Minocha, Subhash C" uniqKey="Minocha S" first="Subhash C" last="Minocha">Subhash C. Minocha</name></noCountry><country name="États-Unis"><region name="New Hampshire"><name sortKey="Mohapatra, Sridev" sort="Mohapatra, Sridev" uniqKey="Mohapatra S" first="Sridev" last="Mohapatra">Sridev Mohapatra</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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